| 论文题目 | Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp. |
| 论文题目（英文） | Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp. |
| 作者 | Huang, Yanan(1,2);Liu, Helu(1);Zhou, Yang(1);Lu, Zaiqing(3);Pu, Yujin(1,2);Zhang, Haibin(1) |
| 发表年度 | 2024-02-05 |
| 卷 | 894 |
| 期 | |
| 页码 | 9 |
| 期刊名称 | GENE |
| 摘要 | |
| 摘要_英文 | Saponins derived from holothurians have high potential medicinal value. However, the de novo synthesis of the derivatization of triterpenes is still unclear. Oxidative squalene cyclase (OSC) can catalyze 2,3-Oxidosqualene into diverse products that serve as important precursors for triterpene synthesis. However, the function of the OSC gene in Chiridota sp. has not been elucidated. In this study, an OSC gene derived from the deep-sea holothurian Chiridota sp. was cloned and characterized functionally in a yeast system. The open reading frame of the OSC gene was 2086 bp, which encoded 695 amino acids. The Chiridota sp. OSC gene has a similarity of 66.89 % to the OSC of other holothurian species and 63.51 % to that of Acanthaster planci. The phylogenetic tree showed that the echinozoan OSCs clustered together, and then they formed a sister group to fungi and plant homologs. Chiridota sp. OSC catalyzed 2,3-Oxidosqualene into parkeol. Under high pressure, the relative enzymatic activity and stability of cyclase in Chiridota sp. was higher than that in the shallow-sea holothurian Stichopus horrens. The newly cloned OSC of Chiridota sp. provides key information for the interpretation of the saponin synthesis pathway in deep-sea holothurians. |
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